Emerson Thacker
Neuropathic Pain Study After Spinal Cord Injury (SCI)

SchoolCuyahoga Heights High School


ProgramScience


MentorChing-Yi Lin, PhD


DepartmentNeurosciences


Research
Neuropathic Pain Study After Spinal Cord Injury (SCI)
Hypothesis
We hypothesize that a dorsal column crush will induce the development of neuropathic pain by assessment of pain-associated mechanical allodynia and thermal hyperalgesia.
Methodology
After SCI, two behavioral tests are performed. 1) Mechanical Allodynia —sensitivity to non-noxious mechanical stimuli will be tested by assessing the “hind paw withdrawal reflex.” Filaments with sequentially increasing or decreasing force will be used to determine the withdrawal threshold. 2) Thermal Hyperalgesia — withdrawal latency will be tested with a thermal nociceptive stimulus. A movable infrared generator with a timer is positioned directly beneath the plantar surface of the hind paw of a rat.
Outcomes
When exposed to a non-noxious mechanical stimuli, mechanical allodynia starts to develop six weeks post dorsal column crush and will gradually get worse. When exposed to thermal nociceptive stimulus, no thermal hyperalgesia developed over the 6-week observation period after SCI.